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Gov政府機關單位
Thesis論文期刊
- Systemic resistance of plants induced by Bacillus cereus
- Bacillus cereus biocontrol strain was isolated from the rhizosphere of lily plants, which suppresses disease development of Botrytis leaf blight. When applying in the rhizosphere of maize plants, B. cereus suppresses symptom development of Southern leaf blight and promotes plant growth. The interaction of plant and B. cereus results in systemic resistance of plants against fungal pathogens. Several differentially expressed genes have been identified via suppression subtractive hybridization, cDNA-AFLP, and specific PCR amplification, which display differential expression patterns in response to B. cereus treatment and B. elliptica infection with or without B. cereus pre-treatment. In addition, transposon mutagenesis was applied to identify the genes related to ISR elicitation.
- Plant defense-related genes of lily
- In Oriental lily cultivar Star Gazer, the treatments of salicylic acid (SA) and probenazole conduct resistance against Botrytis elliptica; in addition, the infection of lily leaves by B. elliptica induces systemic resistance to B. elliptica in upper leaves. A cDNA, designated LsGRP1, coding for glycine-rich protein was cloned from cv. Star Gazer. The Northern hybridization demonstrates that LsGRP1 gene expression increases in the leaves of lily plants after chemical treatment SA and probenazole, and B. elliptica infection. The promoter assay indicates that LsGRP1 gene is SA, probenazole, and B. elliptica-inducible.
- Chitinase system of Bacillus cereus
- Bacillus cereus 28-9, an antagonistic bacterium against Botrytis elliptica, produces two chitinases, ChiCW and ChiCH. Genes encoding ChiCW and ChiCH have been cloned in E. coli for protein expression and purification. An in vitro assay indicates that ChiCW is an antifungal chitinase, but ChiCH only caused a slight inhibition on spore germination. The antifungal activity of ChiCW requires the chitin-binding domain (CBD) since deletion mutant of ChiCW without CBD lost antifungal activity against B. elliptica.